EHI Peer-Reviewed Journal Publications

EHI Peer-Reviewed Journal Publications (Year 2014-2017)


  1. Ng, L.C., Liew, C.Gutierrez, R., Chong, C.S.Tan, C.H.Yap, G.Wong, P.S.J.Li, M.I. (2017) How Safe is Wolbachiafor Aedes Control? A risk assessment for the use of male Wolbachia-carrying Aedes aegypti for suppression of the Aedes aegypti mosquito population. Epidemiological News Bulletin. 43(1):8-16. [Full Text]
  2. Chau, M.L.Aung, K.T.Hapuarachchi, H.C.Lee, P.S., Lim, P.Y., Kang, J.S., Ng, Y., Yap, H.M., Yuk, H.G., Gutiérrez, R.A.Ng, L.C. (2017). Microbial survey of ready-to-eat salad ingredients sold at retail reveals the occurrence and the persistence of Listeria monocytogenes Sequence Types 2 and 87 in pre-packed smoked salmon. BMC Microbiology; 17(1):46. [Full Text]
  3. Pompon, J., Morales-Vargas, R., Manuel, M., Tan, C.H., Vial, T., Tan, J.H., Sessions, O.M., Vasconcelos, P.D.C., Ng, L.C., Missé, D. (2017). A Zika virus from America is more efficiently transmitted than an Asian virus by Aedes aegyptimosquitoes from Asia. Scientific Reports; 7(1):1215. [Full Text]
  4. Kalimuddin, S., Chen, S.L., Lim, C.T.K., Koh, T.H., Tan, T.Y., Kam, M., Wong, C.W., Mehershahi, K.S., Chau, M.L.Ng, L.C., Tang, W.Y., Badaruddin, H., Teo, J., Apisarnthanarak, A., Suwantarat, N., Ip, M., Holden, M.T.G., Hsu, L.Y., Barkham, T.; Singapore Group B Streptococcus Consortium. (2017). 2015 Epidemic of severe Streptococcus agalactiae Sequence Type 283 infections in Singapore associated with the consumption of raw freshwater fish: a detailed analysis of clinical, epidemiological, and bacterial sequencing data. Clinical Infectious Diseases; 64(suppl_2):S145-S152. [Full Text]
  5. Tan, C.H., Wong, P.J., Li, M.I., Yang, H., Ng, L.C., O'Neill, S.L. (2017). wMel limits zika and chikungunya virus infection in a Singapore Wolbachia-introgressed Ae. aegypti strain, wMel-Sg. PLOS Neglected Tropical Diseases; 11(5):e0005496. [Full Text]
  6. Corbel, V., Fonseca, D.M., Weetman, D., Pinto, J., Achee, N.L., Chandre, F., Coulibaly, M.B., Dusfour, I., Grieco, J., Juntarajumnong, W., Lenhart, A., Martins, A.J., Moyes, C., Ng, L.C., Raghavendra, K., Vatandoost, H., Vontas, J., Muller, P., Kasai, S., Fouque, F., Velayudhan, R., Durot, C., David, J.P. (2017). International workshop on insecticide resistance in vectors of arboviruses, December 2016, Rio de Janeiro, Brazil. Parasites & Vectors; 10(1): 278. [Full Text]
  7. Moyes, C.L., Vontas, J., Martins, A.J., Ng, L.C., Koou, S.Y., Dusfour, I., Raghavendra, K., Pinto, J., Corbel, V., David, J.P., Weetman, D. (2017). Contemporary status of insecticide resistance in the major Aedes vectors of arboviruses infecting humans. PLOS Neglected Tropical Diseases; 11(7):e0005625. [Full Text]
  8. Chau, M.L., Chen, S.L., Yap, M., Hartantyo, S.H.P., Chiew, P.K.T., Wong, W.K., Fong, R.K.H., Tan, W.L., Tan, B.Z.Y., Ng, Y., Aung, K.T., Mehershahi, K.S., Goh, C., Kang, J.S.L., Fernandez, C.J., Barkham, T., Leong, A.O.K., Gutiérrez, R.A., Ng, L.C. (2017). Investigation of a human Group B streptococcus outbreak highlights the importance of proper sourcing and handling of fish for raw consumption. Emerging Infectious Diseases; 12:1982.  [Full Text]

  9. Pang, S.C., Andolina, C., Malleret, B., Christensen, P.R., LamPhua, S.G., Aliff, M., Chong, C.S., Li, D., Chu, S., Russell, B., Rénia, L., Ng, L.C. and Nosten, F. (2017). Singapore’s Anopheles sinensis Form A is susceptible to Plasmodium vivax isolates from the western Thailand–Myanmar border. Malaria Journal; 16:465. [Full Text]

  10. Ang, L.W., Kam, Y.W., Lin, C., Krishnan, P.U., Tay, J., Ng, L.C., James, L., Lee, V.J.M., Goh, K.T., Ng, L.F.P., Tzer, R.P.L. (2017). Seroprevalence of antibodies against chikungunya virus in Singapore resident adult population. PLOS Neglected Tropical Diseases; EMID:11cb27b1c5325b79. [Full Text]

  11. Tien, W.P., Lim, G., Yeo, G., Chiang, S.N., Chong, C.S., Ng, L.C., Hapuarachchi, H.C. (2017) SYBR green-based one step quantitative real-time polymerase chain reaction assay for the detection of Zika virus in field-caught mosquitoes. Parasites and Vectors; 10: 427. [Full Text]

  12. Aung, K.T., Hsu, L.Y., Koh, T.H., Hapuarachchi, H.C., Chau, M.L., Gutiérrez, R.A., Ng, L.C. (2017). Prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in retail food in Singapore. Antimicrobial Resistance and Infectious Control; 6:94. [Full Text]

  13. Chau, M.L., Chen, S.L., Yap, M., Hartantyo, S.H.P., Chiew, P.K.T., Fernandez, C.J.,Wong, W.K., Fong, R.K.H., Tan, W.L., Tan, B.Z.Y., Ng, Y., Aung, K.T., Mehershahi, K.S., Goh, C., Kang, J.S.L., Barkham, T., Leong, A.O.K., Gutiérrez, R.A., Ng, L.C. (2017). Group B Streptococcus infections caused by improper sourcing and handing of fish for raw consumption, Singapore, 2015 – 2016. Emerging Infectious Diseases; 23(12): 1982. [Full Text]

  14. Singapore Zika Study Group, 2017, Outbreak of Zika virus infection in Singapore: an epidemiological, entomological, virological, and clinical analysis. The Lancet Infectious Diseases 17(8): 813-821. [Full Text]


  1. Chau M.L., Hartantyo S.H.P., Yap M., Kang J.S.L., Aung K.T., Gutiérrez R. A., Ng L.C., Tam C.C., Barkham T. (2016). Diarrheagenic pathogens in adults attending a hospital in Singapore. BMC Infectious Diseases. 16(1): 32. [Full Text]
  2. Hapuarachchi, H.C., Koo, C., Kek, R., Xu, H., Lai, Y.L., Liu, L Kok, S.Y., Shi, Y., Chuen, R.L.T., Lee, K.S., Maurer-Stroh, S., Ng, L. C. (2016). Intra-epidemic evolutionary dynamics of a Dengue virus type 1 population reveal mutant spectra that correlate with disease transmission. Scientific Reports. 6:22592. [Full Text]
  3. Yap, G., Liang, S.H., Hapuarachchi, H.C., Chong, C.S., Tan, L.K., Liu, X., Yuan, S., Zeng, K., Koo, C., Cook, A., Lai, Y.L. & Ng, L.C. (2016)Dengue Outlook for Singapore in 2016. MOH’s Epidemiological News Bulletin. 42(1): 3-10. [PDF]
  4. Ng, L.C., Tan, H.K, Tan, L.K., Chong, C.S. & Ho, D. (2016) Evolving Dengue Control Programme in Singapore. MOH’s Epidemiological News Bulletin. 42(1): 11-15. [PDF]
  5. Nandar, K., See, W., Zhang, E., Oh, O., Yap, G., Lai, Y.L., Mailepessov, D., Tan, L.K., Ng, L.C., Ho, D., Tey, J., Cutter, J., Lyn, J., Heng, D. (2016). Risk of Yellow Fever Importation into Singapore. MOH’s Epidemiological News Bulletin.42(2): 43-48. [PDF]
  6. Tan, C.H., Wong, P.S., Li, M.Z., Yang, H.T., Chong, C.S., Lee, L.K., Yuan, S., Leo, Y.S., Ng, L.C., Lye, D.C. (2016). Membrane feeding of dengue patient's blood as a substitute for direct skin feeding in studying Aedes-dengue virus interaction. Parasites and Vectors. 9(1): 211. [Full Text]
  7. Yung, C.F., Chong, C.Y., Yeo, K.T., Liew, C., Ng, L.C., Tan, N.W., Yeo, G.S.H, Tee, N.W., Lin, R.T., Tan, T.C., Rajadurai, V.S., Chan, J.K., Thoon, K.C. (2016). Zika Virus: An Evolving Public Health Threat. Annals of the Academy of Medicine, Singapore. 45(4): 148-151. [PDF]
  8. Soh, L.T., Squires, R.C., Tan, L.K., Pok, K.Y., Yang, H., Liew, C., Shah, A.S., Aaskov, J., Abubakar, S., Hasabe, F., Ng, L.C., Konings, F. (2016). External quality assessment of dengue and chikungunya diagnostics in the Asia Pacific region, 2015. Western Pacific Surveillance and Response Journal; 7(2). [Full Text]
  9. Hapuarachchi, H.C.Koo, C., Rajarethinam, J., Chong, C.S., Lin, C.Yap, G.Liu, L., Lai, Y.L., Ooi, P.L., Cutter, J., Ng, L.C. (2016). Epidemic resurgence of dengue fever in Singapore in 2013-2014: A virological and entomological perspective. BMC Infectious Disease. 16(1): 300. [PDF]
  10. Koo, C., Kaur, S., Teh, Z.Y., Xu, H., Nasir, A., Lai, Y.L., Khan, E., Ng, L.C., Hapuarachchi, H.C. Genetic Variability in Probe Binding Regions Explains False Negative Results of a Molecular Assay for the Detection of Dengue Virus. Vector Borne and Zoonotic Diseases. 16(7): 489-495. [Epub]
  11. Yuan, S., Liu, X., Kok, S.Y., Rajarethinam, J., Liang, S.H., Yap, G., Chong, C.S., Lee, K.S., Tan, S.Y.S., Chin, K.Y.C., Lo, A., Kong, W., Ng, L.C., Cook, A.R. (2016). Three-Month Real-Time Dengue Forecast Models: An Early Warning System for Outbreak Alerts and Policy Decision Support in Singapore. Environmental Health Perspectives. [Full Text]
  12. Chen, H., Parimelalagan, M., Takei, F., Hapuarachchi, H.C., Koay, E.S., Ng, L.C., Ho, P.S., Nakatani, K., Chu, J.J. (2016). Development of 2, 7-Diamino-1, 8-Naphthyridine (DANP) Anchored Hairpin Primers for RT-PCR Detection of Chikungunya Virus Infection. PLoS Neglected Tropical Diseases; 10(8): e0004887. [Full Text]
  13. Ng, L.C., Cook, A.R., Yap, G., Ho, D. (2016). Use of Prediction Models for Risk Analysis and Decision-Making in Public Health—The Catch-22 Conundrum. Annals, Academy of Medicine; 45(8): 364-346. [PDF]
  14. Corbel, V., Achee, N.L., Chandre, F., Coulibaly, M.B., Dusfour, I., Fonseca, D.M., Grieco, J., Juntarajumnong, W., Lenhart, A., Martins, A.J., Moyeis, C., Ng, L.C., Pinto, J., Raghavendra, K., Vatandoost, H., Vontas, J., Weetman, D., Fouque, F., Velayudhan, R., David, J.P. (2016). Tracking Insecticide Resistance in Mosquito Vectors of Arboviruses: The Worldwide Insecticide resistance Network (WIN). PLoS Neglected Tropical Diseases; 10(12): e0005054. [Full Text]


  1. Kam, Y.W., Pok, K.Y., Tan, L.K., Eng, K.R., Hapuarachchi, H.C., Leo, Y.S., Ng, L.C., Ng, L.F.P. (2015). Sero-prevalence and cross-reactivity of chikungunya virus specific anti-E2EP3 antibodies in arbovirus-infected patients. PLoS Neglected Tropical Diseases; 9(1): e3445. [Full Text]
  2. Teng, T.S., Kam, Y.W., Lee, B., Hapuarachchi, H.C., Wimal, A., Ng, L.C., Ng, L.F.P. (2015). A systematic meta-analysis of immune signatures in acute chikungunya virus-infected patients. Journal of Infectious Diseases; 211(12): 1925. [Full Text]
  3. Hapuarachchi, H.C., Chua, R.C.R., Thein, T.L., Lee, L.K., Lee, K.S., Lye, D.C., Ng, L.C., Leo, Y.S. (2015). Clinical outcomes and genetic differences within a monophyletic Dengue virus type 2 population. PLoS One; 10(3):e0121696.  [Full Text]
  4. Yung, C.F., Lee, K-S., Thein, T.L., Tan, L.K., Gan, V.C, Wong, J., Lye, D.C, Ng, L.C., Leo, Y.S. (2015). Dengue serotype-specific differences in clinical manifestation, laboratory parameters and risk of severe disease in adults, Singapore. American Journal of Tropical Medicine & Hygiene; 92(5): 999. [Full Text]
  5. Ng, L.C., Chem, Y.K., Koo, C., Rose, N.B.M., Faridah, M.A., Lee, K.S., Chong, C.K. (2015). Dengue outbreaks in Singapore and Malaysia caused by different viral strains. American Journal of Tropical Medicine & Hygiene; 92(6): 1150. [Full Text]
  6. Low, S.L., Lam, S., Wong, W.Y., Teo, D., Ng, L.C., Tan, L.K. (2015). Dengue seroprevalence of healthy adults in Singapore: Sero-survey among blood donors, 2009. American Journal of Tropical Medicine & Hygiene; 93(1): 40. [Full Text]
  7. Pang, S.C., Chiang, L.P., Tan, C.H., Vythilingam, I., Lam-Phua, S.G., Ng, L.C. (2015). Low efficacy of delthamethrin-treated net against Singapore Aedes aegypti is associated with kdr-type resistance. Tropical Biomedicine; 32(1): 140. [Full Text]
  8. Pok, K.Y., Squires, R.C., Tan, L.K., Takasaki, T., Abubakar, S., Hasebe, F., Partridge, J., Lee, C.K., Lo, J., Aaskov, J., Ng, L.C., Koning, F. (2015). First round of external quality assessment of dengue diagnostics in the WHO Western Pacific Region, 2013. Western Pacific Surveillance and Response Journal; 6(2). [Full Text]
  9. Van Panhuisa, W.G., Choisy, M., Xiong, Xa, Chok, N.S., Akarasewid, P., Iamsirithawornd, S., Lam, S.K., Chong, C.K., Lam, F.C., Phommasakh, B., Vongphrachanhi, P., Bouaphanh, K., Rekol, H., Hien, N.T., Thaik, P.Q.,  Duong, T.N. Chuang, J.H., Liu, Y.L., Ng, L.C.Shi, Y., Tayag, E.A., Roque Jr, V.G., Lyndon Suy, L.L.L., Jarman, R.G., Gibbons, R.V., Velasco, J.M.S., Yoon, I.K., Burke, D.S., and Cummings, D.A.T. (2015).  Region-wide synchrony and traveling waves of dengue across eight countries in Southeast Asia.  Proceedings of the National Academy of Sciences.  112(42): 13069. [Full Text]
  10. Huixin Chen, H., Parimelalagan, M., Lai, Y.L., Lee, K.S., Evelyn Siew, R., Koay, C., Hapuarachchi, H.C., Ng, L.C., Ho, P.S., and Chu, J.J.H.  (2015). Development and Evaluation of a SYBR Green-Based Real-Time Multiplex RT-PCR Assay for Simultaneous Detection and Serotyping of Dengue and Chikungunya Viruses.  Journal of Molecular Diagnostics.  17(6): 722. [Full Text]
  11. Yuan, S., Liu, X., Kok, S.Y., Rajarethinam, J., Liang, S.H., Yap, G., Chong, C.S., Lee, K.S., Tan, S.Y.S., Chin, K.Y.C., Lo, A., Kong, W., Ng, L.C., and Cook, A.R. Three-Month Real-Time Dengue Forecast Models: An Early Warning System for Outbreak Alerts and Policy Decision Support in Singapore. Environmental Health Perspectives.doi:10.1289/ehp.1509981. [Epub]    


  1. Kumar, P., Pok K.Y.Tan, L.K., Chow, A., Leo Y.S., Ng, L.C. (2014). Development and evaluation of baculovirus-expressed Chikungunya virus E1 envelope proteins for serodiagnosis of Chikungunya infection. Journal of Virological Methods; 206: 67-75. [Full Text]
  2. Kasai, K., Komagata, O., Itokawa, K., Shono, Toshio., Ng, L.C., Kobayashi, M., Tomita, T. (2014). Mechanisms of pyrethroid resistance in the dengue mosquito vector, Aedes aegypti: target site insensitivity, penetration, and metabolism. PLOS Neglected Tropical Diseases;8(6): e2948. [Full Text]
  3. Kek, R., Hapuarachchi H.C., Chiew-Yuan Chung, Humaidi, M., Razak, A., Chiang, S., Lee, C., Tan, C.H., Yap, G., Chong, C.S., Lee, K.S., Ng, L.C. (2014). Feeding host range of Aedes albopictus (Diptera: Culicidae) demonstrates its opportunistic host-seeking behavior in rural Singapore. Journal of Medical Entomology;51(4):880-883. [Full Text]
  4. Toh, Y.X., Gan, V., Balakrishnan, T., Zuest, R., Poidinger, M., Wilson, S., Appanna, R., Thein, T.L., Ong, A., Ng, L.C., Leo Y.S., Fink, K. (2014). Dengue serotype cross-reactive, anti-E protein antibodies confound specific immune memory for 1 year after infection. Frontiers in Immunology; 5: 388. [Full Text]
  5. Lee, R., Choong, C.T.H., Goh, B.P.L., Ng, L.C., Lam-Phua, S.G. (2014). Bioassay and biochemical studies of the status of pirimiphos-methyl and cypermethrin resistance in Aedes (Stegomyia) aegypti and Aedes (Stegomyia) albopictus (Diptera: Culicidae) in Singapore. Tropical Biomedicine; 31(4): 670-679. [PDF]
  6. Goh, V., Yap, H.M., Gutierrez, R.A., Ng, L.C., Vesper, S.S. (2014). DNA-based analyses of molds in Singapore public buildings results in a proposed Singapore environmental relative moldiness Index. Tropical Biomedicine; 31(4): 663-669. [PDF]
  7. Koou, S.Y., Chong, C.S., Vythilingam, I., Lee, C.Y., Ng, L.C. (2014). Insecticide resistance and its underlying mechanisms in field populations of Aedes aegypti adults (Diptera: Culicidae) in Singapore. Parasites & Vectors;7: 471. [Full Text]
  8. Chan, A., Chiang, L.P., Hapuarachchi H.C., Tan, C.H., Pang, S.C., Lee, R., Lee, K.S., Ng, L.C., Lam-Phua, S.G. (2014).DNA barcoding: complementing morphological identification of mosquito species in Singapore. Journal of Parasites & Vectors; 7(1): 569. [Full Text]
  9. Oon, L., Ng, L.C. (2014).Chikungunya in Singapore – the battle continues. Annals, Academy of Medicine; 43(6): 325-327. [PDF]
  10. Xu, H.Y., Fu, X.J., Lee, L.K.H., Ma, S., Goh, K.T., Wong, J.C., Habibullah, M.S., Lee, G.K.K., Lim, T.K., Tambyah, P.A., Lim, C.L., Ng, L.C. (2014). Statistical modeling reveals the effect of absolute humidity on dengue in Singapore. PLOS Neglected Tropical Disease; 8(5): e2805. [Full Text]
  11. Low, J.G., Sung, C., Wijaya, L., Wei, Y., Rathore, A.P., Watanabe, S., Tan, B.H., Toh, L., Chua, L.T., Hou, Y., Chow, A., Howe, S., Chan, W.K., Tan, K.H., Chung, J.S., Cherng, B.P., Lye, D.C., Tambayah, P.A., Ng, L.C., Connolly, J., Hibberd, M.L., Leo, Y.S., Cheung, Y.B., Ooi, E.E., Vasudevan, S.G. (2014). Efficacy and safety of celgosivir in patients with dengue fever (CELADEN): a phase 1b, randomised, double-blind, placebo-controlled, proof-of-concept trial. Lancet Infectious Diseases; 14(8): 706-715. [Full Text]
  12. Gan, C.V., Tan, L.K., Lye, C.D., Pok, K.Y., Mok, S.Q., Chua, C.R., Leo, Y.S, Ng, L.C. (2014). Diagnosing dengue at the point-of-care: utility of a rapid combined diagnostic kit in Singapore. PLoS Neglected Tropical Diseases; 9(3): e90037. [Full Text]
  13. Gutierrez, R. (2014). Guidance on hygiene and safety in the food retail sector. FAO RAP Publication 2014/16. Available from: (Published as an outcome of the FAO-NEA Regional Workshop on hygiene and safety in food retail, 12-14 Sep 2013).